Molecular Cloning of a Gene Affecting the Autolysin Level and Flagellation in Bacillus subtilis
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چکیده
منابع مشابه
Nucleotide sequences of the Bacillus subtilis flaD locus and a B. licheniformis homologue affecting the autolysin level and flagellation.
A DNA fragment containing the flaD locus of Bacillus subtilis, which had been cloned into plasmid pAC3, was subcloned into an M13 phage and sequenced. The sequence contained five open reading frames (ORFs), of which ORF2 was the flaD gene. Unexpectedly, the sequence of the flaD locus was identical to that of sin [sporulation inhibition gene; Gaur, N. K., Dubnau, E. & Smith, I. (1986). Journal o...
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To evaluate the role of wild promoter of L-phenylalanine dehydrogenase (PheDH) gene, referred to as pdh, from Bacillus sphaericus in expression, cloning of pdh gene in Bacillus subtilis was performed. The whole pdh gene was cloned in pHY300PLK shuttle vector and amplified, construct (pHYDH) then transformed in B. subtilis ISW1214 and E. coli JM109. The pdh endogenous promoter presented no effec...
متن کاملIn vitro elaboration Mutagenesis and cloning of the PA gene in Bacillus subtilis
Background: The immune antigen of Bacillus anthracis is a protein that can attach to the surface receptor of all human cells. At the surface of cancer cells, there is a receptor that activates the uPA (Urokinase plasminogen) that do not exist in normal human cells. Objectives: The aim of this study was changing the location of the attachment of the PA gene by a dir...
متن کاملIn vitro elaboration Mutagenesis and cloning of the PA gene in Bacillus subtilis
Background: The immune antigen of Bacillus anthracis is a protein that can attach to the surface receptor of all human cells. At the surface of cancer cells, there is a receptor that activates the uPA (Urokinase plasminogen) that do not exist in normal human cells. Objectives: The aim of this study was changing the location of the attachment of the PA gene by a dir...
متن کاملGenetic control of flagellation in Bacillus subtilis.
Flagellation in Bacillus subtilis was shown to involve at least three loci: a gene H controlling the specificity of the flagellar antigen; a gene fla controlling the presence or absence of flagella; and a gene mot controlling the function of the flagella. The loci were shown to be nonallelic and unlinked in transformation tests. Strains W 23 and SB 108-b, a derivative of strain 168, were shown ...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1988
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-134-6-1611